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. 2015 May 12;4:113. [Version 1] doi: 10.12688/f1000research.6370.1

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Copyright: © 2015 Rollo BN et al.

This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).

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Supplemental Figure S2. — Following tissue digestion, single cells were fluorescently labelled with HNK1 and HNK1/NCAM and cells sorted by FACS. ( A) Scatter plots show typical results of sorting embryonic tissue from quail embryonic day 5 (QE5), QE8, and chicken embryonic day 9 (ChE9). The typical percentage yields of HNK1 ^+ve cells are shown following FACS of single cells from the midgut and dorsal root ganglia (DRG). ( B) QE5 MG HNK1 ^+ve cells plated on laminin show the characteristic multipolar morphology of ENC cells. ( C) QE8 midguts were digested and single cells fluorescently labelled with HNK1 (Alexa 488) and NCAM (Alexa 647). The scatter plot shows that the majority of NCAM ^+ve cells also express HNK1. NCAM ^+ve/HNK1 ^+ve are able to be differentially sorted from the NCAM ^-ve/HNK1 ^+ve population.