Supplemental Figure S3. Qualitative (q) PCR of HNK1 sorted cells.

To assess the enrichment for ENC markers following FACS for HNK1, QE8 midgut-derived cells were assessed for expression of SOX10 (a NC marker) and Hu-D (a neuronal marker). Cell populations analysed by qPCR were cells which were not sorted (pre-sort), sorted cells which were not HNK1 ^+ve (-ve sort) and HNK1 ^+ve sorted cells (HNK1). HNK1 ^+ve cells showed a significant increase in expression of the markers tested when compared with pre-sorted cells indicating successful enrichment of ENC cells by FACS.