Figure 6. Formation of aggregates by ENS cells in cell-cell adhesion assays correlates with adhesion molecule expression pattern.

A’ A’’. At 2 h QE8 N-cadherin and NCAM ^+ve ENS cells formed small aggregates (mixed Hu ^+ve neurons and SoxE ^+ve ENC cells) and chains (mostly ENC cells). B. At 6 h spherical aggregates were formed but neurons (HuC/D ^+ve) and ENC cells (SoxE ^+ve) were only partially segregated. C. At 18 h most HuC/D ^+ve neurons formed the centre of each spherical aggregate surrounded by SoxE ^+ve ENC cells. D. NCAM immunoreactivity was low on the external face of SoxE ^+ve ENC cells (indicated by dotted line), but high around HuC/D ^+ve neurons, whether these were located centrally or on the periphery (arrows). E. N-cadherin immunoreactivity was associated with all cells in the aggregate, but was less distinct on the external surface (indicated by dotted line).