| BrdU (bromodeoxy uridine) |
Mouse IgG |
1/100 |
Amersham BU-1 |
| DNA (nuclei) |
DAPI |
0.5 μM |
LifeTechnol. |
| HNK-1 (3-sulphoglucuronosyl antigen) |
Mouse IgM |
1/20 |
MCRI |
| HuC/D (neuronal Elav family) |
Mouse IgG |
1/200 |
Molecular Probes 16A11 |
| HU (neuronal Elav family) |
Human IgG |
1/6000 |
Dr. Vanda Lennon, Mayo Clinic, MN |
| NAPA-73 (neurofil.-assoc. prot., 73 kDa) |
Mouse IgM |
1/20 |
DSHB E/C8 |
| N-cadherin |
Mouse IgG |
1/100 |
Sigma GC-4 |
| N-cadherin |
Rabbit IgG |
1/200 |
Abcam ab18203 |
| NCAM |
Rabbit IgG |
1/200 |
Chemicon AB5032 |
| NCAM extracellular |
Mouse IgG |
1/100 |
DSHB 5e |
| Neurofilament M |
Rabbit IgG |
1/100 |
Chemicon AB1987 |
| QCPN (quail not chick perinuclear antigen) |
Mouse IgG |
1/50 |
DSHB |
| SMA (smooth muscle actin) |
Mouse IgG |
1/200 |
Sigma 1A4 |
| SoxE (Sox-8,9 and 10) |
Rabbit IgG |
1/2500 |
Dr Craig Smith, MCRI |
| TuJ-1 (neuron-specific β-III tubulin) |
Mouse IgG |
1/400 |
R&D, MAB1195 |
| Phosphohistone-H3 |
Rabbit IgG |
1/200 |
Upstate #07-081 |
|
Detection probes:
|
| mouse IgGγ: FITC |
Goat IgG |
1/50 |
Jackson Immunores. 115-545-071 |
| mouse IgG: Alexa 350 |
Goat IgG |
1/100 |
Molecular Probes MP A21049 |
| mouse IgG: Alexa 488 |
Goat IgG |
1/400 |
Molecular Probes MP A11029 |
| mouse IgG: Alexa 594 |
Donkey IgG |
1/2000 |
Molecular Probes MP A21203 |
| mouse IgMμ: Texas Red |
Goat IgG |
1/400 |
Jackson Immunores. 115-075-075 |
| mouse IgG: Alexa 647 |
Goat IgG |
1/1000 |
Molecular Probes MP A21235 |
| rat IgG: biotin |
Donkey IgG |
1/100 |
Jackson Immunores. 712 065 153 |
| rabbit IgG: Alexa 488 |
Goat IgG |
1/400 |
Molecular Probes MP A110334 |
| rabbit IgG: Alexa 594 |
Donkey IgG |
1/2000 |
Molecular Probes MP A-21207 |
| rabbit IgG: biotin |
Donkey IgG |
1/100 |
Jackson Immunores. 711 065 152 |
| Biotin (Streptavidin: AMCA) |
NA |
1/500 |
Jackson Immunores. 016 150 084 |
