Table 2.
Total number of reads used in the assembly of the O. faveolata metatranscriptome and those aligned against each of the holobiont components (‘O. faveolata’, ‘Symbiodinium spp.’ and ‘other-eukaryotes’). Percentages are of the total of the raw reads for each row.
raw reads |
aligned reads |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
month | colony condition and no. | total | O. faveolata | % | Symbiodinium spp. | % | other-eukaryotes | % | total | % |
bleaching | ||||||||||
November 2010 | unbleached 1 | 25 263 324 | 18 397 266 | 72.8 | 3 458 682 | 13.7 | 1 760 588 | 7.0 | 23 616 536 | 93.5 |
unbleached 2 | 22 167 068 | 17 899 877 | 80.7 | 2 400 183 | 10.8 | 838 853 | 3.8 | 21 138 913 | 95.4 | |
bleached 1 | 17 619 525 | 15 869 696 | 90.1 | 292 570 | 1.7 | 143 158 | 0.8 | 16 305 424 | 92.5 | |
bleached 2 | 17 321 791 | 15 740 825 | 90.9 | 1 098 670 | 6.3 | 161 552 | 0.9 | 15 902 377 | 91.8 | |
post-bleaching | ||||||||||
March 2011 | unbleached 1 | 45 411 481 | 35 655 303 | 78.5 | 5 944 196 | 13.1 | 1 160 146 | 2.6 | 42 759 645 | 94.2 |
unbleached 2 | 21 592 782 | 15 583 474 | 72.2 | 1 855 259 | 8.6 | 3 018 662 | 14.0 | 204 57395 | 94.7 | |
bleached 1 | 33 446 863 | 25 284 275 | 75.6 | 1 377 763 | 4.1 | 4 268 640 | 12.8 | 30 930 678 | 92.5 | |
bleached 2 | 64 535 106 | 50 983 253 | 79.0 | 4 804 314 | 7.4 | 5 339 275 | 8.3 | 56 322 528 | 87.3 | |
October 2011 | unbleached 1 | 31 158 747 | 22 055 972 | 70.8 | 949 427 | 3.0 | 6 541 857 | 21.0 | 29 547 256 | 94.8 |
unbleached 2 | 50 476 948 | 40 373 490 | 80.0 | 6 881 860 | 13.6 | 1 144 076 | 2.3 | 48 399 426 | 95.9 | |
bleached 1 | 30 226 535 | 21 934 584 | 72.6 | 1 376 168 | 4.6 | 4 297 944 | 14.2 | 26 232 528 | 86.8 | |
bleached 2 | 28 292 342 | 18 843 835 | 66.6 | 1 207 627 | 4.3 | 4 005 816 | 14.2 | 18 843 835 | 66.6 | |
average | 32 292 709 | 24 885 154 | 77.5 | 2 894 993 | 7.6 | 2 606 796 | 8.5 | 29 204 712 | 93.6 |