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. 2015 Feb 10;3(2):e00119. doi: 10.1002/prp2.119

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© 2015 The Authors. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Immunoblot of GFP or GFP-tagged B₂R in total extracts of HEK 293 cells that stably express B₂R-GFP. Cells maintained in their serum-containing culture medium were treated for 3 h as indicated. Bottom: Scanning intensity of relevant bands in replicated experiments. ANOVA showed that B₂R-GFP bands significantly varied among groups (P < 10^-4) and also that free GFP values were significantly heterogeneous (P < 0.01). *P < 0.01 versus values of control cells (second set of histograms from the left; Dunnett's test).