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. 2015 Mar 12;3(2):e00123. doi: 10.1002/prp2.123

Figure 3.

Figure 3

Cytosensor measurements in HEK–hP2X7 cells overexpressing P2X7 and parental HEK293 cells. (A) Concentration-dependent stimulation of HEK–hP2X7 cells (solid lines, black circles) and parental HEK293 cells (dotted lines, inverted triangles) by BzATP as analyzed using a cytosensor system. Parental HEK293 cells and human HEK–hP2X7 cells were compared with respect to respiration and metabolic activity (acidification). Respiratory inhibition in HEK–hP2X7 cells at 100 μmol/L BzATP was indicative of cellular toxicity. Level of significance: *P < 0.05, **P < 0.01, ***P < 0.001, n = 4. (B) Cell impedance as a function of stimulation of P2X7 using BzATP. Curve was fitted to HEK–hP2X7 signals (solid line, black circles) using an enzyme kinetic model. Parental HEK293 cells were used as a control (inverted triangles). Level of significance: *P < 0.05, **P < 0.01, n = 4.