Figure 1.

MPD treatment mitigates intestinal inflammation to increase mouse survival from lethal colitis. (A) Diagram of MPD treatment protocol upon DSS-induced colitis. (B) Intestinal inflammation was induced by 2.5% DSS in mice for acute injury study, mice were then treated with vehicle (sterile saline) or MPD (25 mg/kg) once daily for 5 days starting on day 3. Weight gain and loss were recorded. (C) Intestinal inflammation was induced by 2.5% DSS for 7 days followed by 5 days of water for healing studies. MPD was given to mice at 25 mg/kg daily on day 3 together with DSS for 10 days. Curve of weight gain and loss percentage was showed. Colon lengths were measured after mice were sacrificed on day 12 (D). Disease severity of DSS-induced colitic injury was scored (E). Results are expressed as mean ± SEM (n = 7−8 per group). Data were analyzed using ANOVA, **P < 0.01 versus controls. (F) C57BL/6 mice were orally administrated with 4% DSS in drinking water for 7 days; simultaneously, these mice were i.p. treated with MPD (40 mg/kg) once daily for 5 days. Kaplan–Meier curve was used to estimate the percent survival of DSS-induced colitis with and without MPD intervention, n = 8 each group.