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. 2015 Feb 10;3(2):e00118. doi: 10.1002/prp2.118

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© 2015 The Authors. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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MPD treatment induces apoptosis of inflammatory laminar propria mononuclear cells (LPMC). (A) LPMCs were isolated from the collected surgical specimen from UC patients and cultured for 12 h in the presence or absence of MPD Incubation (200 ng/mL). Frequency of apoptosis in the CD3⁺ or CD11b⁺ LPMCs were determined with 7-AAD and Fluorochrome-labeled AnnexinV as percentage of 7-AAD^-AnnexinV⁺. Representative scatter graphs are shown. (B) LMPCs were isolated from colonic tissues of colitic mice, apoptosis was determined as percentage of 7-AAD^-AnnexinV⁺. Results are expressed as mean ± SEM (n = 5 or 6), *P < 0.05 versus controls. Representative scatter graphs are shown.