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. 2015 Feb 4;2:14056. doi: 10.1038/mtm.2014.56

Figure 1.

Figure 1

Design, expression, and purification of MD11-eIF3f recombinant fusion proteins. (a) Scheme of recombinant MD11-eIF3 constructs containing His tag for affinity purification. Mutations in which C-terminal lysines were replaced to arginines are indicated in MD11-K510R mutant. (b) Protein expression level in E. coli. After bacterial cell lysis, the soluble (S) and the insoluble (P) fractions were analyzed by SDS-PAGE, stained with Coomassie blue and identified by western blotting using an anti-His antibody. (c) After purifications, soluble recombinant fusion proteins were analyzed by SDS-PAGE, stained with Coomassie blue and detected by western blotting with an anti-His antibody and an anti-eIF3f antibody.