Figure 2.

Screening of MD₁₁-wt eIF3f and MD₁₁-K510R cytotoxic effect by ⁵¹Cr-release assay on human and murine cell lines. 10 nmol/l of MD₁₁-wt eIF3f and MD₁₁-K510R were incubated with (a) nontumorigenic and (b) tumor cells and ⁵¹Cr release measurements were performed 0.5, 1, 2, 3, 4, 8, and 18 hours after protein incubation. Doxorubicin (1 µmol/l) was used as toxicity control and protein-free buffer was tested to discard any buffer-linked toxicity. Each data point is the mean of three values ± SD.