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. 2015 Feb 4;2:14056. doi: 10.1038/mtm.2014.56

Table 1. Optimized expression conditions used for recombinant protein production in E. coli system.

Protein E. coli bacterial strain (IPTG) mmol/l Additive
MD11-AN BL21-CodonPlus (DE3)-RIPL (Stratagene) 0.2 0.4 mol/l sucrose
MD11-NF E. coli BL21 (DE3) (Novagen) 0.5 None
MD11-wt BL21-CodonPlus (DE3)-RIPL 0.5 None
MD11-K510R BL21-CodonPlus (DE3)-RIPL 0.2 10 g/l glycerol