| B16, GL26, HEK293E, HeLa |
DMEM with stable glutamine and Na pyruvate (PAA, GE Healthcare), 10% FBS (PAA, GE Healthcare), 1% Penicillin/Streptomycin (Gibco) |
| C2C12 |
DMEM with stable glutamine and Na pyruvate, 15% FBS, 1% Penicillin/Streptomycin |
| MCF7 |
DMEM with stable glutamine and Na pyruvate, 10% FBS, 1% Penicillin/Streptomycin, 10 µg/ml insulin (Sigma), 0.1 mmol/l nonessential amino acids (Gibco) |
| MCF10-2A |
F12 DMEM with stable glutamine and Na pyruvate (Gibco), 10% FBS, 10 µg/ml insulin, 0.5 µg/ml hydrocortisone (Gibco), 100 ng/ml cholera toxin (Sigma), 20 ng/ml epidermal growth factor (Sigma) and 1% Penicillin/Streptomycin |
| HCT116 p53+/+ and HCT116 p53−/−
|
McCoy’s with stable glutamine (PAA, GE Healthcare), 10% FBS, 1% Penicillin/Streptomycin (supplemented with 50 µg/ml G418 (Gibco) for p53−/− cells) |
| Saos-2 |
McCoy’s with stable glutamine, 15% FBS, 1% Penicillin/Streptomycin |
| LN-229, T98G, U87, Colo857 |
RPMI-glutamax (Gibco), 10% FBS, 1% Penicillin/Streptomycin |
| Raji |
RPMI-glutamax, 10% FBS, 1 mmol/l Na pyruvate (Gibco), 0.1 mmol/l nonessential aminoacids, 10 µg/ml gentamicin (Gibco) |
| BJ |
DMEM with stable glutamine and Na pyruvate, 10% FBS, 0.1 mmol/l nonessential aminoacids |
