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. 2015 Jan 14;1:14062. doi: 10.1038/mtm.2014.62

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed. under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Efficiency of triplex DNA (TriD)-mediated purification with the minicircle (MC) products reconstituted with different proportion of impure producing plasmids (PPs). (a) Gel illustration of MC products before and after TriD-mediated purification. About 1-, 2-, 5-, and 10% PP5 were added into MCs made using PP2 or PP5 before purification. Each lane loaded with 1 μg of EcoR1-restricted MC product. (b) Amount of impure DNAs in the MC products as determined by qPCR before and after purification. ^c,dP < 0.05; ^a,bP < 0.01.