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. 2015 Feb 18;2:14067. doi: 10.1038/mtm.2014.67

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Overexpression of Serca2a in hES2C-CMs increases the expression of calcium homeostasis genes and improves calcium cycling. (a) Protein expression of cardiac-specific markers by differentiated cells. Key calcium cycling proteins Serca2a, PLN and NCX1 are expressed highly in hES2C-CMs and at significant levels in hiPS31.3C-CMs, compared to human heart, whereas they are absent from the cell lines they were derived from. Error bars depict ± standard error mean (SEM) (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). (b) Adenoviral overexpression of Serca2a in hES2C-CMs leads to increase in protein expression of cardiac specific markers. Serca2a and EGFP as a control were adenovirally overexpressed in hES2C-CMs. The AdSerca2a infected cells showed higher expression of not only Serca2a, but also PLN and NCX1. Error bars depict ± SEM (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). (c) Effect of Serca2a overexpression on calcium cycling in hES2C-CMs. Adenoviral overexpression of Serca2a in hES2C-CM improved calcium cycling as shown by the significant increase in the peak height of the calcium transients as well as the time to reach the peak height. Error bars depict ± SEM (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001).