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. 2015 May 29;10(5):e0126561. doi: 10.1371/journal.pone.0126561

Fig 4. The increased mRNA and protein levels of oxygenases HMOX1 and COX2 induced by hyperosmotic media in HCECs.

Fig 4

Primary HCECs exposed to media at 312, 400 and 450 mOsM for 4 hours were lysed for mRNA expression by RT-qPCR (A), and the cells treated for 24 hours were performed for immunofluorescent staining (B) or lysed in RIPA buffer for Western blotting (C), to determine the mRNA expression and protein production of HMOX1 and COX2. Data showing mean±SD, n = 5, * P<0.05, ** P<0.01, compared with 312 mOsM normal control.