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. 2015 Jan 22;9(6):1043–1055. doi: 10.1016/j.molonc.2015.01.004

Figure 7.

Figure 7

RhoC regulates PC3 cancer cell retention in the lungs and experimental metastasis formation in vivo. (A) Representative 2D image used to monitor the ratio between siRNA control‐transfected YFP‐PC3 and RhoC‐depleted CFP‐PC3 cells. Scale bar, 200 μm. (B) Number of cells in the lungs. 60 random independent fields were analysed and cell number was scored from 3 independent experiments. Data are expressed as % of total cells analysed. Values are means ± SEM; **p < 0.01. (C) Effects of RhoC depletion on experimental metastasis formation. Mice were injected intravenously with PC3 cells transfected with control siRNA or RhoC‐1 siRNA. Quantification of metastatic foci on the lung surface (8 mice for control, 5 mice for RhoC siRNA). Each dot represents foci from one mouse; lines and numbers indicate means; **p < 0.01.