Fig 4. Testosterone production by Sickle and Hemi mouse Leydig cells is impaired.

In vitro testosterone production from Leydig cells isolated from WT, Hemi, and Sickle mouse testis. Cells were stimulated, or not, with LH (0.5 ng/ml and 10 ng/ml), dbcAMP (1 mM), 22-hydroxycholesterol (25 μM; 22-HC), and pregnenolone (25 μM, P5). Each bar represents the mean ± SEM. *P < 0.05 vs WT. n = 7–13.