Fig 6. Oxidative stress markers are increased in the testis of Sickle and Hemi mice.
Protein expressions of NADPH oxidase subunit gp91phox (A), oxidative stress marker 4-HNE (B), and antioxidant enzyme GPx-1 (C) in the testis of WT, Hemi, and Sickle mice were measured by Western immunoblot. The analysis of 4-HNE is a densitometric composite of all proteins in each lane [26, 27]. Upper panels are representative Western immunoblots. Lower panels represent quantitative analyses of the proteins in the same groups. Each bar represents the mean ± SEM. *P < 0.05 vs WT. n = 8–16. 4-HNE = 4-hydroxy-2-nonenal; Gpx-1 = glutathione peroxidase-1.