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. 2015 May 29;10(5):e0127511. doi: 10.1371/journal.pone.0127511

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© 2015 Tachtatzis et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — Immunoperoxidase staining using cell cycle phase-specific antibodies in a representative case of chronic HBV infection A—E. Immunofluorescence showing the relation between HBcAg expression and Mcm-2 (F) and cyclin A (G). A: Mcm-2 identifies cells that have entered the cycle. B: Cyclin D1, expression is maximal during G1. C: Cyclin A is expressed maximally during S phase. D: Cytoplasmic expression of Cyclin B1 during G2, nuclear and cytoplasmic expression. E: Phosphorylated histone 3 protein expressed in mitosis. F: Immunofluorescence for Mcm-2 (green) and HBcAg (red), showing frequent co-localisation (yellow). G: Immunofluorescence for cyclin A (green) and HBcAg (red). Co-localisation was never seen.