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. 2015 May 29;11(5):e1005255. doi: 10.1371/journal.pgen.1005255

Fig 1. CD177 gene structure and HNA-2 expression.

Fig 1

A). Structures of CD177 gene and pseudogene at chromosome 19q13.31 region. CD177 gene with nine exons and its pseudogene with six pseudo-exons are separated by 8,407 nucleotides. CD177 pseudogene is highly homologous to CD177 between exon 4 and 9. Vertical arrow indicates the CNV assay probe location. B). HNA-2 expression varies in different donors. Characteristic light-scatter properties were used to identify neutrophils in flow cytometry. Neutrophils from different donors vary in the percentages of HNA-2 positive cells. Right panel shows the absence of HNA-2 expression on neutrophils from a donor. C). Amplification of full-length CD177 cDNA from HNA-2 deficient donors. All HNA-2 deficiency donors expressed full-length CD177 mRNA the full-length CD177 cDNAs (1,411 bps) were detected in RT-PCR.