Fig 4. Androgen mediated regeneration of the prostate epithelium after castration does not depend on rapidly serially proliferating progenitor/TA cells.

Representative images of triple immunofluorescence staining for CIdU, IdU and Krt14 on sections of the distal/intermediate (A, C) and the proximal (B, D) regions of ducts from the dorsal prostate of castrated mice sequentially treated with CIdU and IdU (1 day each) at day 2 (A, B) or day 3 (C, D) after androgen supplementation. In all experiments, mice were sacrificed immediately after the end of IdU administration. Yellow arrowheads indicate double-labeled cells while insets show that the majority of the cells are single labeled. (E): Graphic representation of the percentages of epithelial prostate cells (both basal and luminal) labeled with CIdU, IdU, or CIdU/IdU (DL) for the indicated treatment groups. 2dR and 3dR indicate mice that were treated with the thymidine analogs at day 2 or day 3 after androgen supplementation, respectively. The predicted stochastic fraction is also shown. Data represent the means ± SD for three mice per group. n indicates the average number of nuclei counted per mouse. * indicates p<0.05.