Fig 2. Assessment of βAR desensitization in non-asthmatic ASM and asthmatic ASM cells.

a, To determine whether βAR are differentially phosphorylated in non-asthmatic ASM and asthmatic ASM cells, the cells were plated on cover slips and β₂AR phosphorylation was visualized by confocal microscopy using anti-phospho-S-355/356 β₂AR antibody (green) (Scale-100 μM) (n = 5/group). b, Plasma membranes isolated from non-asthmatic ASM and asthmatic ASM cells were subjected to ^[125]I-CYP (cyanopindalol) βAR binding at saturation concentration of 250 pmol. *p<0.005 vs. non-asthmatic ASM, (n = 7/group). c, To investigate changes in desensitization βAR components lysates (100 μg) from non-asthmatic ASM and asthmatic ASM cells were immunoblotted for ubiquitously expressed GRKS, GRK2, 3, 5 or 6. The blots were stripped for each probing. Furthermore, the blots were immunoblotted for β-arrestin 1 and 2. Actin was blotted as loading control (n = 7/group).