Fig 3. Treatment with 4-MP attenuated CCl₄-induced liver fibrosis in mice.

Fibrosis was induced with serial administrations of CCl₄ and the animals were concurrently treated with 4-MP for 2 weeks. (A) Serum levels of ALT, IL-6, MCP-1, and TNF-α. (B) Sirius red staining for collagen (scale bar = 500 μm). (C) Immunohistochemical staining for α-SMA demonstrating activated HSCs (scale bar = 500 μm). (D) Western blotting for α-SMA, TGF-β1, ADH3, and CYP2E1 and the respective quantification of proteins. The data are expressed as mean ± SEM. *P < 0.05, **P < 0.01 compared with the respective controls.