Fig 5. Treatment with 4-MP increased production of IFN-γ by NK cells in CCl₄-treated mice.

Hepatic fibrosis was induced with CCl₄ and the mice were concurrently treated with 4-MP for 2 weeks. (A and B) FACS analyses of isolated mononuclear cells to assess the frequency of NK cells and IFN-γ production, respectively. (C) Serum levels of IFN-γ. (D) qPCR for IFN-γ in isolated NK cells. (E) Fluorescent TUNEL staining to demonstrate apoptosis in activated HSCs after treatment with 4-MP and the respective quantified data (scale bar = 25 μm). The data are representative of three independent experiments and are presented as mean ± SEM. *P < 0.05, **P < 0.01 when compared with the respective controls.