Fig 2. ND-13 peptide attenuated toxic-induced cell death in primary neuronal cultures.

Primary neuronal cultures were prepared from c57/bl6 mice brains, as elaborated in the methods section. Cells were exposed to increasing doses of hydrogen peroxide (H2O2, 0–50 μM) (A), 6-OHDA (0–25 μM) (B) for 24 hours. ND-13 (4 μM) or vehicle were applied 1 hour before the toxic insults. (A) ND-13 prevented H2O2-induced cell death and (B) 6-OHDA-induced cell death, measured by LDH cytotoxicity assay. * P<0.05 as compared to control cells exposed to vehicle. The experiments were repeated 3 times in triplicates.