Fig 2. HFD led to impairment of IRS-1/Akt pathway and activation of inflammatory responses in primary hepatocytes.

Primary hepatocytes were isolated from mice fed a normal diet or HFD for 12 weeks (as described in the Materials and Methods section). (A) Representative western blots of IRS-1/Akt pathway proteins and the respective densitometry measurements; (B) Representative western blots of inflammatory proteins and the corresponding densitometry measurements. Data presented as mean ± SD, n ≥3 mice/group, *p < 0.05, ** p < 0.01 versus control group.