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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Nat Genet. 2015 Apr 20;47(6):677–681. doi: 10.1038/ng.3269

Figure 4. Inactivation of the PHP domain renders mycobacteria sensitive to nucleoside analogues.

Figure 4

(A) Primer extension analysis performed as in Figure 1H in the presence of 200 μM of the adenosine analog ara-A. Incorporation of ara-A impedes primer extension. Whereas wild-type DnaE1MTB can excise ara-A and resume DNA synthesis, the PHP mutants cannot.

(B) Determination of the minimum inhibitory concentration (MIC) of ara-A for the indicated M. smegmatis strains. Pink color indicates cellular respiration; blue color indicates lack of respiration.