Figure 5. Mechanotransduction for cell compaction-dependent collagen VI synthesis.
Cells were cultured for 16 h on circular FN islands (500 μm diameter) at low or high plating density (0.2 or 2.4 × 105 cells/cm2, respectively) with or without various inhibitors and activators of mechanical signaling and Col VI mRNA levels were measured. Modulators include (A) anti-Integrin alpha V neutralizing Ab (INTG Ab: 100 μg/ml), pertussis toxin (P-toxin; 25 ng/ml), gadolinium (500 μM), Anti-N-cadherin neutralizing Ab (N-cad Ab: 200 μg/ml) and L-NAME (2 μM); (B) cytochalasin D (CytD: 2.5 μM), C3 (2 μg/ml), Y27632 (10 μM), cytotoxic necrotizing factor (CNF1: 100 ng/ml), ML-7 (10 μM), NSC23766 (50 μM) and CN02 (0.2 U/ml); (C): H89 (10 μM), C1 (10 μM), PMA (100 nM), LY294002 (10 μM), U0126 (10 μM), SB203580 (500 nM), SP600125 (100 nM), PP1 (10 μM) and SB431542 (1 μM); (D): SR11302 (1 μM) and mithramycin (250 nM). *p < 0.01.