(A) Enhancer trap constructs used to generate all lines. (B1-B Max) To convey as
much information as possible, compound microscopic images were taken at multiple depths
(B1–B4) and used to generate Maximum Intensity Projections (B Max) in which signal
from multiple focal depths is visible. Arrowheads mark the olfactory placode (blue),
spinal neurons (green), otic vesicle and retina (orange) and epithelial cells (yellow).
Images B1-B Max are lateral views. (C–F) Confocal images enhance fine details
relative to compound microscopic images. Glial endfeet are clearly visible in the confocal
image (D, Arrowhead) but are not clear in the compound scope image (C, Arrowhead).
Habenulae are visible in confocal (F, Orange arrowheads) but not compound (E, Orange
arrowheads) image. (E,F) Pineal gland marked by yellow arrowhead. Images C–F are
dorso-lateral views. Scale bars: B1-B MAX 250um. C, D 250um. E, F 100um.