Figure 6. Intu^Dtm embryos exhibit reduced ossification and misaligned ribs.

(A) Ossification within the phalanges (red arrows) is delayed by ~one day in Intu^Dtm mutant embryos compared to wild type at E16.5, E17.5, and E18.5. (B) Ossification of vertebrae of Intu^Dtm embryos is reduced and does not extend as caudally as in wild type mice at E16.5, but this difference is resolved by E17.5 and E18.5. Red arrowheads indicate the most caudal vertebra with ossification. (C) The extent of endochondral ossification in long bones (humerus, radius, and ulna) is decreased in Intu^Dtm mutants as quantified by measuring the size of the ossified region (red) as compared to the full length of the long bone (between joints). *p<0.05, **p<0.005. (D) Longitudinal sections of E14.5 wild type and Intu^Dtm mutant ulna. Red arrows mark the hypertrophic zones (hyp) and yellow arrows mark the columnar/proliferative zones (col). (E) Intu^Dtm mice exhibit delayed sternum ossification and rib misalignment. Ventral views of sterna with attached ribs isolated from wild type and Intu^Dtm mutant embryos at E17.5 and E18.5. Asterisks indicate the extra attached rib on one side of the sternum in Intu^Dtm mutants.