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. 2015 May 28;4:10.3402/jev.v4.27369. doi: 10.3402/jev.v4.27369

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© 2015 Inés Lozano-Ramos et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Urine EVs are eluted in low protein containing SEC fractions.

In panel a, a representative analyses of a SEC processed sample is shown. In each fraction (indicated on the x-axis), the expression of CD9 and CD63 and the total protein content were determined. The left axis shows the total protein content (mg/mL), whereas the right axis shows the median fluorescence intensity (MFI) data for CD9 and CD63. The isotype control for flow cytometry assay is depicted by a dotted line.

In panel b, fractions showing the highest MFI value for each CD marker (CD9 square symbols and CD63 triangle symbols) were grouped (n=10). Fractions in which protein content was first detected are also shown (circles, n=8). Higher CD9 and CD63 MFI values were routinely detected between fractions 7 and 10, well before any protein elution was detectable.