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. 2015 Feb 15;5(3):1000–1016.

Figure 4.

Figure 4

HM910 deceased CDK4 protein levels via a shortened protein half-life. A. Protein turn-over assay of CDK4 after HM910 treatment. NCI-H929 cells were pre-incubated with CHX (10 mg/mL), for 2 h. Next, NCI-H929 cells were treated with HM910 (5 μmol/L). Every six hours, cells were harvested and protein levels assessed using Western blotting. GAPDH was used as a loading control. B. Gray value analysis of CDK4 protein turn-over was determined by Image J. The CDK4 protein half-life was carried out by densitometric analysis. CDK4 protein half-life decreased from 38.6 to 15.7 h. C. The mRNA levels of CDK4 as determined by Real-time quantitative PCR. GAPDH expression was used to normalize data.