Figure 6.

HM910 increased reactive oxygen species (ROS) generation, and induced loss of mitochondrial membrane potential (ΔΨm) in NCI-H929 cells. A & B. Both HM910 and topotecan induced significant increases in ROS generation, in NCI-H929 cells. Representative results (left) are shown, similar results were obtained in two other independent trials. Data (right) shown are means ± SD for independent determinations in triplicate. C & D. Both HM910 and topotecan reduced mitochondrial transmembrane potential. Representative results (left) are shown, similar results were obtained in two other independent trials. Data (right) are presented as mean ± SD from three independent experiments (*, P < 0.05; **, P < 0.01). E. NAC, an anti-oxidant, could inhibit cell apoptosis induced by HM910.