Figure 7.

HM910 induced MM cell apoptosis through mitochondrial dependent pathway. A. Apoptosis related changes in protein levels. In both HM910 and topotecan treatment groups expression of Bcl-2 and Bcl-xl were down regulated, and Bad and Bim protein levels were up regulated, however, no change Bax protein levels was observed. B and C. The averaged relative expression levels of Bcl-2 protein family in NCI-H929 cells in the presence of increasing concentrations of HM910 or topotecan. Values are presented as mean ± SD calculated from three independent experiments (*, P < 0.05; **, P < 0.01). D. Changes in mitochondrial dependent apoptotic pathway related proteins. Cytochrome c released from mitochondria to cytoplasm increased, as did activation of caspases 9, 3 and PARP cleavage were observed in a concentration dependent manner. E and F. The averaged relative expression levels of caspase in NCI-H929 cells in the presence of increasing concentrations of HM910 or topotecan. Representative blots from three independent experiments are shown (*, P < 0.05; **, P < 0.01).