Figure 1.

Expression of Wnt2 in normal tissue and non-small cell lung cancers. (A) expression of Wnt2 in 8 representative pairs of NSCLC lung cancer tissues (T) and adjacent normal lung (N) tissues detected by RT-PCR (top) and Western blot (bottom). The results were processed with ImageJ (B). (C) immunohistochemical staining of Wnt2 protein in 5 representative pairs of histologic types of NSCLC (ADC, BAC, ASC, SCC and LCC) and adjacent normal lung tissues. (D) examples are shown for absent, low, median and high Wnt2 expression in NSCLC cancer tissues. (E) distribution of Wnt2 immunohistochemical results in 264 NSCLC patients as well as that according to pathological subtypes (ADC, SCC, LCC, ASC and others). Kaplan–Meier analysis of overall survival (F) and relapse-free survival (G) of patients with NSCLC (P = 0.002 by log-rank test) according to expression of Wnt2 protein. Vertical bars on survival curves indicate censored cases.