Figure 5.

rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P < 0.05. B: Representative immunofluorescence images demonstrate the translocation process of β-catenin from the membrane to nucleus in H460 cells incubated with rWnt2 but not with IgG control at a concentration of 200 ng/mL at the indicated time points. Antibody against β-catenin was labelled with green color and nuclei were labelled with blue color (DAPI). Original magnification, 400 ×; scale bars, 50 um.