Figure 5.

HIF-1α inhibition is involved in the prevention of hypoxia-induced EMT by hispidulin. HT-29 cells were incubated under hypoxic conditions in the presence or absence of hispidulin (25 μM). A. Hispidulin suppressed the hypoxia-induced expression of HIF-1α. B. Silencing of HIF-1α with shRNA restored hypoxia-induced reduction in E-cadherin expression. C. Overexpression of HIF-1α partially blocked the inhibitory effect of hispidulin on hypoxia-induced E-cadherin expression. The immunoblots represent three independent experiments. Data are presented as mean ± SD from three independent experiments. *P < 0.05 vs. control, ^P < 0.05 vs. hypoxia, **P < 0.05 vs. hypoxia + His.