Figure 4.

Effect of TADs on SMMC-7721 cell cycle and cycle-related molecules. Cells were treated with TADS for 48 h and stained with PI. The cells were then analyzed by FACs. (A) TADs arrested SMMC-7721 cell cycle at the G₁ phase. (a) Control. (b) 6.25 μM. (c) 12.50 μM. (d) 25.00 μM. (B) Quantitation data of (A). Data were expressed as percentage of cell population in G₀-G₁, S and G₂-M phases of the cell cycle (n = 3). (C) Effect of TADs on cycle proteins expression of Cyclin D1, Cyclin E, Cyclin B1 and Cdc2 by western blot analysis. (D) Quantitation data of (C). TADs decreased the protein expressions of Cyclin E, Cyclin B and Cdc2 expressions, and increased Cyclin D1 expression in a dose-dependent manner compared to the untreated control. Data were expressed as means ± SD (n = 3). *p < 0.05, **p < 0.01 vs. the control group.