Table 2.
The sense primer and antisense primer used to amplify the MSI2, β-catenin, LEF-1 and TCF-4 gene in Quantitative Real-time PCR were shown
| Gene name | Sense primer | Size (bp) |
|---|---|---|
|
| ||
| Antisense primer | ||
| MSI2 | 5’TTCGCAGACCCAGCAAGTG 3’ | 154 |
| 5’TCGCAGATAACCCGCCTAC 3’ | ||
| β-catenin | 5’GGTTTCCCATTGGTTCAC 3’ | 246 |
| 5’CATAAATCCCGCCTAACG 3’ | ||
| LEF-1 | 5’CTCTGTCTTTCCTGCTGTTG 3’ | 215 |
| 5’CTAAATCGCCTTCCTCTTCG 3’ | ||
| TCF-4 | 5’CAGTCTTCCTCCGATGTC 3’ | 115 |
| 5’CCCGCTTCCTCTATTTGC 3’ |
bp base pair; LEF-1 Lymphoid enhancer factor-1; TCF-4 T cell transcription factor-4.