Figure 1.

Bladder fibroblasts promote BCa cells invasion. To test how bladder fibroblasts affect BCa cells invasion, those 2 types of cells were co-incubated in a transwell setting. Fibroblasts were seeded in the top well of 6-wells transwell plate and three BCa cell lines, the T24, UMUC3, or 5637 cells, were seeded in the bottom transwells (pore size: 0.4 μm). After 2 days of co-culture, BCa cells were re-seeded on matrigel coated transwells (pore size: 8 μm) to determine their invasion rate. All invaded cells were counted in 6 independent fields (magnitude, 200X). Invaded BCa cells were calculated and averaged. BCa cells with fibroblasts co-culture were compared to BCa without fibroblasts co-culture (control). All data were repeated in three independent experiments and analyzed by t-test.