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. 2015 Feb 15;5(3):1146–1157.

Figure 4.

Figure 4

Fibroblasts enhance BCa cell invasion via modulation of BCa IL-6 expression. A. IL-6 expression was increased in both UMUC3 and 5637 cells after co-culture with fibroblasts. We screened some altered metastasis-related genes in BCa cells after co-culture with fibroblasts. Q-PCR results revealed that IL-6 expression was increased in both UMUC3 and 5637 cells after co-culture with fibroblasts and IL-8 expression was increased in only UMUC3 cells after co-culture with fibroblasts. B. Increase of ERα expression in fibroblasts further increased fibroblasts-enhanced IL-6 expression in BCa 5637 cells, suggesting fibroblast ERα may be able to function through modulation of the IL-6 expression to influence the BCa cell invasion. C. Blocking IL-6 with neutralizing antibody led to partially reverse the fibroblasts-enhanced BCa cell invasion. BCa cells were seeded in the transwell pre-coated with martrigel, fibroblasts were seeded on the bottom well, IL-6 anti-body (1:400 dilution) or mock control was added to the co-culture wells for 24 hrs. to determine the invasion rate.