Table 1. Primer sequences and amplification conditions.
Gene | Primer sequence | Amplicon size (bp) | Ta | Number of cycles |
---|---|---|---|---|
β-actin | 5’ - gcc aga gcg gga gtg gtg aa - 3’ | 309 | 53°C | 29 |
5’ - ggc ttg ggc tca ggg tca tt - 3’ | ||||
c-fos | 5’ - gcc tcg ttc ctc cag tcc ga - 3’ | 434 | 53°C | 32 |
5’ - tgc gat gga aag gcc agc cc - 3’ | ||||
c-jun | 5’ - acc ttc aac acc cca gcc atg - 3’ | 554 | 55°C | 23 |
5’ - ggc cat ctc ttg ctc gaa gtc - 3’ |
Ta=annealing temperature
bp=base pair