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. 2015 May;70(5):373–379. doi: 10.6061/clinics/2015(05)12

Table 1. Primer sequences and amplification conditions.

Gene Primer sequence Amplicon size (bp) Ta Number of cycles
β-actin 5’ - gcc aga gcg gga gtg gtg aa - 3’ 309 53°C 29
5’ - ggc ttg ggc tca ggg tca tt - 3’
c-fos 5’ - gcc tcg ttc ctc cag tcc ga - 3’ 434 53°C 32
5’ - tgc gat gga aag gcc agc cc - 3’
c-jun 5’ - acc ttc aac acc cca gcc atg - 3’ 554 55°C 23
5’ - ggc cat ctc ttg ctc gaa gtc - 3’

Ta=annealing temperature

bp=base pair