Fig. 2. GYY4137 increases plasma H₂S and cystathionine-γ-lyase (CSE) activity but decreases CSE mRNA in the myocardium after I/R.

A: Plasma H₂S concentration measured by chemical method (n = 6-9). B: Myocardial CSE activity (n = 6-10). C: CSE mRNA expression determined by quantitative real-time PCR (n = 5). Sham: sham operation; Vehicle+I/R: saline pre-treatment for 7 days followed by 30 minutes of myocardial ischemia and 24 hours of reperfusion, G12.5+I/R, G25+I/R or G50+I/R: GYY4137 pre-treatment at 12.5 mg/(kg·day), 25 mg/(kg·day) or 50 mg/(kg·day) for 7 days followed by 30 minutes of myocardial ischemia and 24 hours of reperfusion. ^*P<0.05, ^**P<0.01.