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. 2015 May 16;15:121. doi: 10.1186/s12870-015-0479-4

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© Florez et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Arabidopsis overexpressing TcBBM leads to spontaneous regeneration from the cotyledon A, D. E12-Ω-CaMV-35S::TcBBM (BBM-N) Arabidopsis line showing spontaneous regeneration of cotyledon like structures from the seedling cotyledons (black arrows). B. E12-Ω-CaMV-35S::TcBBM (BBM-CD) Arabidopsis line showing no phenotype. C. Arabidopsis Col 0 wild type. E. The corresponding TcBBM levels of the three E12-Ω-CaMV-35S::TcBBM lines shown in images A, B and C. Expression levels were analyzed by RT-qPCR and the TcBBM gene normalized relative to AtPP2a and AtUBQ10. Image scale bars = 1 mm.