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. 2015 May 16;15:121. doi: 10.1186/s12870-015-0479-4

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© Florez et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TcBBM overexpression in cacao leads to spontaneous direct somatic embryogenesis. A. 35S::TcBBM cacao embryo over-expressing TcBBM going through the process of spontaneous direct somatic embryogenesis. B. Further development of same E12-Ω-CaMV-35S::TcBBM cacao embryo (14 days after image on A). C. E12-Ω-CaMV-35S::TcBBM explant after 14 days of being subjected to hormone induced somatic embryogenesis. D. SCA6 wild-type cacao embryo showing normal cotyledon development and no spontaneous embryo regeneration. Image scale bars = 1 mm.