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Analysis of DNA binding of GmWRP1 recombinant proteins. (A) Sequences of the Pchn0 probe, which contains two TTGACC W-box sequences, and the mPchn0 probe, which has the TTGACC sequences mutated into TTGAaC. (B) Sequence-specific binding of Pchn0 by Arabidopsis AtWRKY33 (AtW33) but not soybean GmWRP1 recombinant proteins. In mPchn0, changes in the TTGACC sequences to TTGAaC resulted in complete abolishment of binding by AtWRKY33.
