Fig. 5.

Reduced callose signals in 15 DPA seed transfer cells (TCs) in GhKOR1-down-regulated transgenic cotton. Resin-embedded seed cross-sections cut at 15 DPA were stained with Aniline Blue for callose. (A) Image of the WT seed section. Note the strong callose signal (arrows) in the TCs in comparison with the buffer-only control (insert). The latter displayed no fluorescent signals in the TCs. (B–D) Images of the seed sections of RNAi line1, RNAi line5, and Co-S line1, respectively. Note that the callose signal in the TCs in these three lines was abolished entirely in comparison with the WT (A) and buffer-only control (insert in A). en, endosperm; isc, inner seed coat; tc, transfer cell, indicated by the white triangle. Scale bars=20 μm in A–D.