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. 2015 Apr 6;66(11):3189–3199. doi: 10.1093/jxb/erv128

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Silencing of PUB17 in N. benthamiana or tobacco compromises Cf4/Avr4 cell death but not INF1 cell death. (A) Graph shows the percentage HR elicited by INF1 or Cf4/Avr4 on N. benthamiana plants silenced for NbPUB17 using the TRV-ARM and NC constructs, respectively. Statistical analysis was carried out using ANOVA with pairwise comparisons performed with a Holm–Sidak test; **P ≤0.01 (n=11), error bars show standard error. (B) Representative leaves silenced for NbPUB17 and TRV-GFP showing INF1 HR on the left side of the leaf and Cf4/Avr4 HR on the right. (C) Graph shows the percentage HR elicited by INF1 or Cf4/Avr4 on N. tabacum plants silenced for NtPUB17 (lines 242H, E, and J) are shown beside the non-silenced control Cf9. Statistical analysis was carried out using ANOVA with pairwise comparisons performed with a Holm–Sidak test; **P ≤0.01, ***P ≤0.001 (n=22), error bars show standard error. (D) Representative leaves silenced for NtPUB17 and the control showing INF1 HR on the left side of the leaf and Cf4/Avr4 HR on the right.