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. 2015 Apr 6;66(11):3189–3199. doi: 10.1093/jxb/erv128

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — GFP-StPUB17 and GFP-NtPUB17 strongly accumulate in the nucleolus in planta. (A) Representative CB157 N. benthamiana leaves expressing mRFP-H2B examined by confocal microscopy 48h after infiltration of 35S-GFP-StPUB17, indicating green (left), red (right), and merged (centre) channels, with close-up nuclear confocal images inset. (B) Representative close-up nuclear confocal image showing the sub-nuclear localization of GFP-NtPUB17. Nu indicates the nucleolus and the scale bar is 10 μm. (C) Western blots probed with a GFP antibody showing stable protein fusions of potato and tobacco GFP-PUB17 of the expected size. The lower panels show Ponceau staining of the membrane as a loading control.